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pgem3 plasmid  (Qiagen)


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    Structured Review

    Qiagen pgem3 plasmid
    DNA binding ability of SNBP obtained from: control 1 ( a ); son ( b ) and father ( c ) analyzed by EMSA on 1% agarose gel. Bands on gel representing the state of <t>pGEM3</t> plasmid DNA incubated in a w/w ratio with increasing amount of SNBP from samples containing: CP/Hr ( a ); nCP/Hr ( b ); only–H ( c ). Panel a from supplementary material of Lettieri et al., 2020 . L-group: low impact area group; EMSA: electrophoresis mobility shift Assay; SNBP: sperm nuclear basic proteins; CP/Hr: canonical protamines/histones ratio; nCP/Hr: not canonical protamines/histones ratio; only-H: only histones and other basic proteins.
    Pgem3 Plasmid, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgem3+plasmid/pgem3+plasmid/pmc07555199-183-0-19
    Average 90 stars, based on 1 article reviews
    pgem3 plasmid - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Molecular Alterations in Spermatozoa of a Family Case Living in the Land of Fires—A First Look at Possible Transgenerational Effects of Pollutants"

    Article Title: Molecular Alterations in Spermatozoa of a Family Case Living in the Land of Fires—A First Look at Possible Transgenerational Effects of Pollutants

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms21186710

    DNA binding ability of SNBP obtained from: control 1 ( a ); son ( b ) and father ( c ) analyzed by EMSA on 1% agarose gel. Bands on gel representing the state of pGEM3 plasmid DNA incubated in a w/w ratio with increasing amount of SNBP from samples containing: CP/Hr ( a ); nCP/Hr ( b ); only–H ( c ). Panel a from supplementary material of Lettieri et al., 2020 . L-group: low impact area group; EMSA: electrophoresis mobility shift Assay; SNBP: sperm nuclear basic proteins; CP/Hr: canonical protamines/histones ratio; nCP/Hr: not canonical protamines/histones ratio; only-H: only histones and other basic proteins.
    Figure Legend Snippet: DNA binding ability of SNBP obtained from: control 1 ( a ); son ( b ) and father ( c ) analyzed by EMSA on 1% agarose gel. Bands on gel representing the state of pGEM3 plasmid DNA incubated in a w/w ratio with increasing amount of SNBP from samples containing: CP/Hr ( a ); nCP/Hr ( b ); only–H ( c ). Panel a from supplementary material of Lettieri et al., 2020 . L-group: low impact area group; EMSA: electrophoresis mobility shift Assay; SNBP: sperm nuclear basic proteins; CP/Hr: canonical protamines/histones ratio; nCP/Hr: not canonical protamines/histones ratio; only-H: only histones and other basic proteins.

    Techniques Used: Binding Assay, Control, Agarose Gel Electrophoresis, Plasmid Preparation, Incubation, Electrophoresis, Mobility Shift

    Analysis on 1% agarose gel of pGEM3 plamid DNA breakage induced by H 2 O 2 . In the presence of SNBP of the son ( a ) and father ( b ). SNBP: sperm nuclear basic proteins.
    Figure Legend Snippet: Analysis on 1% agarose gel of pGEM3 plamid DNA breakage induced by H 2 O 2 . In the presence of SNBP of the son ( a ) and father ( b ). SNBP: sperm nuclear basic proteins.

    Techniques Used: Agarose Gel Electrophoresis

    DNA protection analysis on 1% agarose gel of pGEM3 plasmid DNA in the presence of increasing (0.4, 0.6 and 0.8) controls (lanes 6, 9 and 12), son (lanes 4, 7 and 10) and father (5, 8 and 11) SNBP/DNA ratios. SNBP: sperm nuclear basic proteins.
    Figure Legend Snippet: DNA protection analysis on 1% agarose gel of pGEM3 plasmid DNA in the presence of increasing (0.4, 0.6 and 0.8) controls (lanes 6, 9 and 12), son (lanes 4, 7 and 10) and father (5, 8 and 11) SNBP/DNA ratios. SNBP: sperm nuclear basic proteins.

    Techniques Used: Agarose Gel Electrophoresis, Plasmid Preparation

    Related Articles

    Plasmid Preparation:

    Article Title: Molecular Alterations in Spermatozoa of a Family Case Living in the Land of Fires—A First Look at Possible Transgenerational Effects of Pollutants
    Article Snippet: .. pGEM3 plasmid (2867 bp) was extracted from transformed Escherichia coli HB 101 cells, using the standard protocol of the QIAGEN Plasmid Midi Purification kit (QIAGEN Plasmid Midi Purification handbook, third edition© 2020, Hilden, Germany), but following the precautions described in Carbone et al., 2012 [ ], in order to obtain high amounts of supercoiled pDNA. ..

    Transformation Assay:

    Article Title: Molecular Alterations in Spermatozoa of a Family Case Living in the Land of Fires—A First Look at Possible Transgenerational Effects of Pollutants
    Article Snippet: .. pGEM3 plasmid (2867 bp) was extracted from transformed Escherichia coli HB 101 cells, using the standard protocol of the QIAGEN Plasmid Midi Purification kit (QIAGEN Plasmid Midi Purification handbook, third edition© 2020, Hilden, Germany), but following the precautions described in Carbone et al., 2012 [ ], in order to obtain high amounts of supercoiled pDNA. ..

    Purification:

    Article Title: Molecular Alterations in Spermatozoa of a Family Case Living in the Land of Fires—A First Look at Possible Transgenerational Effects of Pollutants
    Article Snippet: .. pGEM3 plasmid (2867 bp) was extracted from transformed Escherichia coli HB 101 cells, using the standard protocol of the QIAGEN Plasmid Midi Purification kit (QIAGEN Plasmid Midi Purification handbook, third edition© 2020, Hilden, Germany), but following the precautions described in Carbone et al., 2012 [ ], in order to obtain high amounts of supercoiled pDNA. ..



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    DNA binding ability of SNBP obtained from: control 1 ( a ); son ( b ) and father ( c ) analyzed by EMSA on 1% agarose gel. Bands on gel representing the state of <t>pGEM3</t> plasmid DNA incubated in a w/w ratio with increasing amount of SNBP from samples containing: CP/Hr ( a ); nCP/Hr ( b ); only–H ( c ). Panel a from supplementary material of Lettieri et al., 2020 . L-group: low impact area group; EMSA: electrophoresis mobility shift Assay; SNBP: sperm nuclear basic proteins; CP/Hr: canonical protamines/histones ratio; nCP/Hr: not canonical protamines/histones ratio; only-H: only histones and other basic proteins.
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    DNA binding ability of SNBP obtained from: control 1 ( a ); son ( b ) and father ( c ) analyzed by EMSA on 1% agarose gel. Bands on gel representing the state of <t>pGEM3</t> plasmid DNA incubated in a w/w ratio with increasing amount of SNBP from samples containing: CP/Hr ( a ); nCP/Hr ( b ); only–H ( c ). Panel a from supplementary material of Lettieri et al., 2020 . L-group: low impact area group; EMSA: electrophoresis mobility shift Assay; SNBP: sperm nuclear basic proteins; CP/Hr: canonical protamines/histones ratio; nCP/Hr: not canonical protamines/histones ratio; only-H: only histones and other basic proteins.
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    Image Search Results


    DNA binding ability of SNBP obtained from: control 1 ( a ); son ( b ) and father ( c ) analyzed by EMSA on 1% agarose gel. Bands on gel representing the state of pGEM3 plasmid DNA incubated in a w/w ratio with increasing amount of SNBP from samples containing: CP/Hr ( a ); nCP/Hr ( b ); only–H ( c ). Panel a from supplementary material of Lettieri et al., 2020 . L-group: low impact area group; EMSA: electrophoresis mobility shift Assay; SNBP: sperm nuclear basic proteins; CP/Hr: canonical protamines/histones ratio; nCP/Hr: not canonical protamines/histones ratio; only-H: only histones and other basic proteins.

    Journal: International Journal of Molecular Sciences

    Article Title: Molecular Alterations in Spermatozoa of a Family Case Living in the Land of Fires—A First Look at Possible Transgenerational Effects of Pollutants

    doi: 10.3390/ijms21186710

    Figure Lengend Snippet: DNA binding ability of SNBP obtained from: control 1 ( a ); son ( b ) and father ( c ) analyzed by EMSA on 1% agarose gel. Bands on gel representing the state of pGEM3 plasmid DNA incubated in a w/w ratio with increasing amount of SNBP from samples containing: CP/Hr ( a ); nCP/Hr ( b ); only–H ( c ). Panel a from supplementary material of Lettieri et al., 2020 . L-group: low impact area group; EMSA: electrophoresis mobility shift Assay; SNBP: sperm nuclear basic proteins; CP/Hr: canonical protamines/histones ratio; nCP/Hr: not canonical protamines/histones ratio; only-H: only histones and other basic proteins.

    Article Snippet: pGEM3 plasmid (2867 bp) was extracted from transformed Escherichia coli HB 101 cells, using the standard protocol of the QIAGEN Plasmid Midi Purification kit (QIAGEN Plasmid Midi Purification handbook, third edition© 2020, Hilden, Germany), but following the precautions described in Carbone et al., 2012 [ ], in order to obtain high amounts of supercoiled pDNA.

    Techniques: Binding Assay, Control, Agarose Gel Electrophoresis, Plasmid Preparation, Incubation, Electrophoresis, Mobility Shift

    Analysis on 1% agarose gel of pGEM3 plamid DNA breakage induced by H 2 O 2 . In the presence of SNBP of the son ( a ) and father ( b ). SNBP: sperm nuclear basic proteins.

    Journal: International Journal of Molecular Sciences

    Article Title: Molecular Alterations in Spermatozoa of a Family Case Living in the Land of Fires—A First Look at Possible Transgenerational Effects of Pollutants

    doi: 10.3390/ijms21186710

    Figure Lengend Snippet: Analysis on 1% agarose gel of pGEM3 plamid DNA breakage induced by H 2 O 2 . In the presence of SNBP of the son ( a ) and father ( b ). SNBP: sperm nuclear basic proteins.

    Article Snippet: pGEM3 plasmid (2867 bp) was extracted from transformed Escherichia coli HB 101 cells, using the standard protocol of the QIAGEN Plasmid Midi Purification kit (QIAGEN Plasmid Midi Purification handbook, third edition© 2020, Hilden, Germany), but following the precautions described in Carbone et al., 2012 [ ], in order to obtain high amounts of supercoiled pDNA.

    Techniques: Agarose Gel Electrophoresis

    DNA protection analysis on 1% agarose gel of pGEM3 plasmid DNA in the presence of increasing (0.4, 0.6 and 0.8) controls (lanes 6, 9 and 12), son (lanes 4, 7 and 10) and father (5, 8 and 11) SNBP/DNA ratios. SNBP: sperm nuclear basic proteins.

    Journal: International Journal of Molecular Sciences

    Article Title: Molecular Alterations in Spermatozoa of a Family Case Living in the Land of Fires—A First Look at Possible Transgenerational Effects of Pollutants

    doi: 10.3390/ijms21186710

    Figure Lengend Snippet: DNA protection analysis on 1% agarose gel of pGEM3 plasmid DNA in the presence of increasing (0.4, 0.6 and 0.8) controls (lanes 6, 9 and 12), son (lanes 4, 7 and 10) and father (5, 8 and 11) SNBP/DNA ratios. SNBP: sperm nuclear basic proteins.

    Article Snippet: pGEM3 plasmid (2867 bp) was extracted from transformed Escherichia coli HB 101 cells, using the standard protocol of the QIAGEN Plasmid Midi Purification kit (QIAGEN Plasmid Midi Purification handbook, third edition© 2020, Hilden, Germany), but following the precautions described in Carbone et al., 2012 [ ], in order to obtain high amounts of supercoiled pDNA.

    Techniques: Agarose Gel Electrophoresis, Plasmid Preparation